| 1. | Expression of crylac in transgenic tobacco plants was assayed with elisa . the results showed that pinll signal peptide sequence enhanced the expression of crylac protein in transgenic tobacco
荧光显微观察到gfp在植物细胞间隙高效表达， westernblot结果显示crylac蛋白也在植物细胞间隙表达。 |
| 2. | Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco . the results of fluorescent detection showed gfp localization in the apoplast
Elisa结果显示，马铃薯蛋白酶抑制剂基因的信号肽序列使crylac基因在转基因烟草中的表达量显著提高。 |
| 3. | Comparison of the entire derived peptide sequence with other serine protease revealed significant homology , especially with a reported gene from another b . pumilus strain tyo - 67 ( 99 % homology )
将克隆到的碱性蛋白酶基因(命名为ap )编码的成熟蛋白n一端序列与纯化的脱毛蛋白酶n一端序列进行比较，二者完全相同。 |
| 4. | We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0
我们直接将含有4个内含子和自身信号肽的2 . 4kb全长人生长激素基因直接克隆至真核表达载体pcdna3 . 0 ，然后利用脂质体转染家蚕bmn细胞，瞬时表达hgh 。 |
| 5. | To allow secretion of the crylac protein into the intercellular space , potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac
运用pcr技术克隆了马铃薯蛋白酶抑制剂基因的信号肽序列，并将其分别连到crylac 、 gfp基因的5 ’端，构建植物转化载体p3301ubisigac和p3301ubisiggfp 。 |
| 6. | To further understand its mechanism of internalization . methods and results 1 . a new peptide sequence was designed based on the analysis of the molecular composed of the membrane penetrating peptides using the peptool life software
最后在计算机photoshop软件上，测量细胞荧光灰度值，并应用spss软件进行统计分析，研究其穿膜能力与温度、浓度、时间及细胞功能状态之间的关系。 |
| 7. | In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ) , nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence
在用烟草作为生物反应器时，分别将该单链抗体靶向细胞质和内质网。经westernblot分析，靶向细胞质中表达时，可溶单链抗体最高占总的可溶蛋白的0 . 06 。 |
| 8. | ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed
( 3 )翻译后水平通过pcr扩增的方式在t - pa基因5端添加了真核分泌信号肽序列和植物翻译起始共有序列aaca ，在3端添加了内质网定位序列kdel ，构建了植物表达载体pbemt 。 |
| 9. | The quickly developing techniques of biological mass spectrometry ( bio - ms ) in recent years realized the high throughput identification of proteins by determining the accurate mass values of trypsin - digested peptides and the randomly selected peptide sequence tags , and have been successfully used in the studies of protein interactions and post - translational modification such as the phosphorylation
摘要近几年快速发展起来的生物质谱技术，依靠（酶解后肽段）精确质量数测定和随机肽序列标签分析，实现了对蛋白质高通量的鉴定，并被成功地用于蛋白质相互作用和蛋白质磷酸化等翻译后修饰研究。 |
| 10. | 2 specific epitope analysis in m3m4 loop target to determine the b cell epitope of a monoclonal antibody against the m3 - m4 loop of nmdar1 , a random phage displayed dodecapeptide library was screened with the monoclonal antibody mab363 against the m3m4 loop of nmdar1 . after four rounds of biopanning , the peptide sequences of positive phage clones were determined and analyzed by dna sequencing
流式细胞仪测定脾细胞cd4 + 、 cds寸淋巴细胞亚群，间接elisa测定血清thl细胞因子几一2 、 tn下一a和n一y ， th一2细胞因子几一4 、 il巧和几一6的水平，同时检测特异性抗体产生情况。 |
